In this experiment, students will simulate the use of CRISPR‐Cas9 to target a genetic mutation found in a patient suffering from Cystic Fibrosis
Students will develop an understanding of guide RNA (gRNA) design, and use agarose gel electrophoresis to examine pre‐prepared DNA samples after CRISPR treatment
Group Size: For 8 Lab Groups
Storage: Room Temperature Stable
Storage of Ready‐to‐Load QuickStrip™ samples in the refrigerator is recommended
Time Required: Complete in 45 minutesThe kit includes:Instructions,
Ready‐to‐Load QuickStrip™ DNA Samples
UltraSpec‐Agarose™
Electrophoresis Buffer (50X).10X Gel Loading Solution,
FlashBlue™ DNA StainAdditional required equipment & materials (not supplied with the kit):DNA Electrophoresis tank and accessories including casting trays and special power supply
