The HIV test detects HIV infection indirectly using an ELISA test against HIV antibodies in the blood
The test works by taking antibodies from the patient's blood and adding them to a microtiter plate coated with HIV antigen
If HIV antibodies are present in the blood, they will bind to the antigens on the plate
This binding is detected with an enzyme‐linked secondary antibody that causes a colour change upon addition of substrate
In this experiment, your students will perform an ELISA test by coating microtiter plate wells with simulated HIV antigen and then test simulated donor serum for anti‐HIV antibodies
Group Size: for 10 lab groups
Storage: Refrigerator
Time Required: 60 minutesThe kit includes:Full instructions.10X ELISA wash buffer
ELISA dilution buffer
Antigen (lypholized)
Primary antibody (lypholized)
Secondary antibody (lypholized)
ABTS (lypholized)
ABTS reaction buffer
Microtitre plates
Transfer pipettes
Snap‐top microcentrifuge tubes.15ml conical tubesAdditional required materials (not included with the kit):Distilled water
