EDVOTEK Transformation of E-Coli (GFP) Kit

HE1482336

1

Description
Specifications
  • In this Transformation of E‐Coli Kit, students will explore the biological process of bacterial transformation using E‐Coli and green fluorescent protein plasmid DNA
  • At the end of the activity, students will have experience observing and analysing acquired traits (ampicillin resistance and fluorescence) as exhibited by transformed bacterial cells
  • Group Size: for 10 lab groups
  • Storage: some components require refrigerator and freezer storage upon receipt
  • Time Required: Set‐up ‐ 50 minutes, Incubation ‐ overnight, Transformation ‐ 15 minutesThe kit includes:Full instructions
  • BactoBeads™ E
  • coli GFP Host
  • Supercoiled pFluoroGreen™ plasmid DNA
  • Ampicillin
  • IPTG
  • Calcium Chloride
  • Growth Additive
  • ReadyPour™ Luria Broth Agar (sterile)
  • Luria Broth Medium for Recovery (sterile)
  • Petri Plates (small & large)
  • Plastic microtipped transfer pipettes
  • Wrapped 10ml pipette (sterile)
  • Toothpicks (sterile)
  • Inoculating loops (sterile)
  • Microcentrifuge tubesAdditional required equipment & materials (not supplied with the kit):Automatic micropipette (5‐50�l).2 x Water Bath (B8R06048)
  • Thermometer (B8R05775)
  • Incubator (B8A64397)
  • Pipette Filler (B8A78062)
  • Ice
  • Marker Pens
  • Bunsen Burner
  • Microwave or Hot Plate
  • Hot gloves
  • Long wave UV light
Glove Type Water Resistant
Group 1 Science
Group 2 Biology
Group 3 Structure Function & Living Organisms
Group 4 Disease
Style Code HE1482336