Murashige and Skoog is a base for plant tissue culture media
The mixture is without plant hormones or agar, which should be added as follows:To maintain callus add 5x10‐8g/litre 2,4‐D (weedkiller) and 8g/litre agar
To maintain apical meristem, or for rooting shoot tips, add 0.3mg/litre IAA, 1mg/litre kinetin and 6g/litre of agar